This pilot study sought to determine if serum brain-derived neurotrophic factor may serve as a biomarker for post-stroke depression in subacute stroke patients. The research enrolled 38 individuals who had subacute strokes. The usual rehabilitation programme, which consisted of of physical therapy each day of occupational therapy five days a week, was completed by all participants. During the subacute stroke phase, the rehab period lasted two weeks. Before, one, and two weeks following the normal rehabilitation programme, we examined the blood levels of and matrix metalloproteinase- Additionally, the Geriatric Depression Scale-Short Form was used to evaluate depressed mood in all individuals at three different times. To ascertain the connection between blood BDNF levels and other variables, Pearson correlation analysis was used. F levels and the demonstrated a considerable improvement over the course of the typical rehabilitation treatment. At each time point, the strongly linked with the serum. According to these findings, serum B may be employed as a biomarker for sad mood in subacute stroke patients. To fully understand these findings, however, more research involving larger study groups is required